ccl2 levels  (R&D Systems)

Journal: Journal of molecular and cellular cardiology

Article Title: Mouse model of post-traumatic stress disorder negatively impacts cardiac homeostasis

doi: 10.1016/j.yjmcc.2025.01.011

Figure Lengend Snippet: A) Male PTSD-like mice had an increased trend ( p = 0.0542) in macrophages (Mac-3) in the left ventricle at 4-weeks post-IFS. No differences were observed in the female groups. Male control n = 3; Female control n = 4; Male NR n = 6; Female NR n = 7; Male PTSD-like n = 6; Female PTSD-like n = 5. B) No differences between phenotypes at 4-weeks post-IFS were observed in circulating levels of CCL2. Male control n = 4; Female control n = 4; Male NR n = 3; Female NR n = 6; Male PTSD-like n = 6; Female PTSD-like n = 4. C) Male and female NR and PTSD-like mice had decreased percent area of Ccr2 mRNA expression compared to respective controls. No significant changes were seen in the amounts of Ccr2 + CD206 mRNA colocalization but female NR mice had an increase in Ccr2 + CD86 mRNA colocalization compared to female controls at 4-weeks post-IFS. Male control n = 3; Female control n = 4; Male NR n = 5; Female NR n = 7; Male PTSD-like n = 6; Female PTSD-like n = 5. Multiple group comparisons were analyzed by two-way ANOVA with Bonferroni posttest.

Article Snippet: Assays for MMP-9 activity (Enzo, BML-AK410, 1:5 dilution of plasma, no dilution of insoluble protein fraction of the LV), circulating CCL2 levels (R&D Systems, MJE00B, 1:10 dilution), and circulating cardiac troponin I (Novus Biologicals, NBP3-00456, 1:2 dilution) were performed according to manufacturer instructions.

Techniques: Control, Expressing

Journal: The Journal of Clinical Investigation

Article Title: Induced clustering of SHP2-depleted tumor cells in vascular islands restores sensitivity to MEK/ERK inhibition

doi: 10.1172/JCI181609

Figure Lengend Snippet: ( A ) Expression levels of Il1a , ccl2 , and ccl3 in sorted populations of EGFP + B16F10 cells, EGFP – VE-cadherin + endothelial cells, and EGFP – CD45 + hematopoietic cells as measured by qPCR. The results are expressed as the relative mean ± SEM of Gapdh mRNA levels ( n = 3 experiments). ( B and C ) Representative images (scale bar: 50 μm) ( B ) showing CD45 + cell infiltrates (green, indicated by arrowheads) in control and shSHP2-silenced B16F10 tumors and quantification ( C ). Gray circles: percentage of CD45 + area/unit of tumor area; colored dots: mean/tumor ( n = 5/group). ** P < 0.01, by 2-tailed Student’s t test. ( D and E ) Representative images (scale bar: 50 μm) ( D ) showing F4/80 (green) macrophage infiltration in control and shSHP2-silenced B16F10 tumors (indicated by arrowheads) and quantification ( E ). Gray circles: percentage of F4/80 + area/unit of tumor area; colored dots: mean/tumor ( n = 5/group). ** P < 0.01, by 2-tailed Student’s t test. ( F ) Proposed model of downstream effects from SHP2 silencing in B16F10 melanoma cells leading to the formation of vascular tumor islands and surrounding tumor cell death. Results in panels A , C and E are presented as means± SEM.

Article Snippet: Tumor CCL2 levels were measured with the Mouse CCL2 DuoSet ELISA kit (R&D Systems, mLMJE00B), and the colorimetric reaction was measured at 450 nm using a microplate reader (Imgen Technologies, BMG LABTECH).

Techniques: Expressing, Control

Journal: iScience

Article Title: A dopamine D1-like receptor-specific agonist improves the survival of septic mice

doi: 10.1016/j.isci.2024.109587

Figure Lengend Snippet: SKF suppressed systemic inflammation (A) Determination by an ELISA of the CCL2 (Aa), TNF-α (Ab), and IL-1β (Ac) levels in the peripheral blood collected from normal healthy control mice (CNT), vehicle administered CLP-treated mice (Vcl/CLP), and SKF administered CLP-treated mice (SKF/CLP) 6 h after CLP. N = 4. A one-way ANOVA with Tukey’s multiple comparison test. (B) The number of mononuclear cells infiltrated into the lungs 6 h after CLP. n = 6. A one-way ANOVA with Tukey’s multiple comparison test. (C) Representative H&E-stained lungs of CNT, Vcl/CLP, and SKF/CLP. Micrographs of all H&E-stained specimens ( n = 6) are shown in Figure S1 . (D) mRNA expression encoding TNF-α (Da) and IL-1β (Db) in the lung of CNT, Vcl/CLP, and SKF/CLP mice 6 h after CLP. n = 7. A one-way ANOVA with Tukey’s multiple comparison test. (E) mRNA expression encoding TNF-α (Ea) and IL-1β (Eb) in the lung of CNT, Vcl/CLP, and FD/CLP mice 6 h after CLP. N = 6. Data are expressed as the mean ± SD. A one-way ANOVA with Tukey’s post hoc test. ∗, ∗∗, ∗∗∗, ∗∗∗∗ indicate statistical significance at p < 0.05, 0.01, 0.001, and 0.0001, respectively. Asterisks in red indicate the significant differences against CNT data. All numerical data and the relevant statistical main factors are shown in Table S1 .

Article Snippet: TNF-α, IL-1β, and CCL2 levels in the plasma samples were determined using ELISA kits (Proteintech, Rosemont, IL, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Comparison, Staining, Expressing

Journal: iScience

Article Title: A dopamine D1-like receptor-specific agonist improves the survival of septic mice

doi: 10.1016/j.isci.2024.109587

Figure Lengend Snippet: Effects of SKF and D1-like receptor antagonist SCH on CLP-induced neuroinflammation and circulating leukocytes All data shown here were obtained using samples obtained 12 h after CLP. (A) Representative flow cytometry plots of dissociated frontal cortex cells. Using anti-CD11b and anti-CD45 antibodies, lymphoid cells, blood-borne macrophages, and resident microglia were separated. (B) Flow cytometry analyses of the percentage in the total live cells of CD11b + /CD45 + myeloid cells (Ba) and CD11b − /CD45 + lymphoid cells (Bb). n = 6 or 7. (C) Effects of CLP and SKF on the mRNA expression of TNF-α (Ca), IL-1β (Cb) by sorted microglia. n = 4. (D) Effects of CLP and SKF on the protein expression of TNF-α (Da) and IL-1β (Db) in the hippocampus. (E) Effects of CLP and SKF on the mRNA expression of TNF-α (Ea) and IL-1β (Eb) in the hippocampus. n = 6. (F) Effects of FD on TNF-α (Fa) and IL-1β (Fb) mRNA expression in the frontal cortex. n = 6. (G) Effects of SKF and SKF+SCH (SK/SC) on the mRNA expression of TNF-α (Ga) and IL-1β (Gb), and CCL2 (Gc) in the frontal cortex of CLP-treated mice. See also Figure 2 S. n = 6. (H) Effects of SKF and SK/SC on the CD45 (Ha) and CD11b (Hb) expression by microglia/macrophages as revealed by FACS. n = 6. (I) Effects of SKF and SK/SC on circulating neutrophils (Ia), T cells (Ib), and B cells (Ic). n = 7. Data are expressed as the mean ± SD. A one-way ANOVA with Tukey’s multiple comparison test. ∗, ∗∗, ∗∗∗, ∗∗∗∗ indicate statistical significance at p < 0.05, 0.01, 0.001, and 0.0001, respectively. Asterisks and “ns” in red indicate statistical difference against CNT data. All numerical data and the relevant statistical main factors are shown in Table S1 .

Article Snippet: TNF-α, IL-1β, and CCL2 levels in the plasma samples were determined using ELISA kits (Proteintech, Rosemont, IL, USA).

Techniques: Flow Cytometry, Expressing, Comparison

Journal: iScience

Article Title: A dopamine D1-like receptor-specific agonist improves the survival of septic mice

doi: 10.1016/j.isci.2024.109587

Figure Lengend Snippet:

Article Snippet: TNF-α, IL-1β, and CCL2 levels in the plasma samples were determined using ELISA kits (Proteintech, Rosemont, IL, USA).

Techniques: Recombinant, Saline, H&E Stain, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software, Cell Counting, Microscopy